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Hepatoprotective and neuroprotective effect of polyprenols

Preclinical study.

It is known that, in hepatosis, not only liver is affected by toxins, but also all the internal organs and the central nervous system. Subacute hepatosis was modelled in rats in laboratory conditions, followed by treatment with polyprenols in different doses. Blood and liver indicators improved in the animals, a psycho-activating effect on the brain was observed, as well as other positive effects. As a result, it was concluded that polyprenols have a pronounced hepatoprotective and neuroprotective effect in the wide range of doses.

Introduction

Traditionally, the poisoning with CC14 in rodents (rats and mice) is considered as a model of toxic liver injury. At the same time, CC14, being a universal prooxidant poison, injures all the internal organs and the central nervous system (UHC). In these terms, poisoning with CC14 can be considered as a fairly universal model of injuries of internal organs (dystrophies) and toxic encephalopathy. Many hepatoprotectors may have a neuroprotective effect. These include metaprote (bemitil), vegetable adaptogens, potassium orotate, methyluracil, ropren. The latter is a 95% concentrate of polyprenols, it is derived from pine and spruce needles, it is recommended as a hepatoprotector of vegetable origin. The central effects of the polyprenolic preparation* are not studied enough.

Study Purpose

The purpose of the study was to assess the hepatoprotective and neuroprotective action of polyprenols* on the model of subacute hepatosis induced by CC14 in rats.

Methods

The subacute hepatosis (liver dystrophy) in rats was modelled with a two-fold intraperitoneal injection of CC14 (2 injections of 1 g/kg with an interval of 3 days). The polyprenolic preparation* (2.15-4.3-11.6 mg/kg) was injected intraperitoneally daily for 2 weeks. After a course of polyprenols*, the animal behavior, blood biochemistry indices, liver morphology and exchange of monoamines in the brain were estimated. The comparator was heptral or ademethionine (300 mg/kg) prepared on the basis of lyophilisate for injections or tablets.

Results

The introduction of a hepatotropic poison resulted in the death of 50% of rats. A significant change in the behavior in the tests of "open field", an elevated plus maze, "resident – intruder", Porsolt were noted in the surviving animals. Signs of injuries of internal organs: liver, pancreas, kidneys were recorded in the blood of rats slaughtered on the 16th day of the experiment. The histological study of the liver in rats identified the experimental hepatosis (liver dystrophy) , that was characterized by the development of the hyaline-drop protein dystrophy, small-drop lipodystrophy and mild lymphocytic infiltration of portal tracts. In the brain, CC14 did not cause significant deviations in the exchange of dopamine, noradrenaline and serotonin in the nucleus accumbens, striatum and frontal cortex of the brain. A variety of tests revealed that polyprenols* in the studied doses (2.15-4.3-11.6 mg/kg) normalize behavior of rats interfered with the introduction of CC14, improve blood and liver indicators in rats with subacute hepatosis and have a psycho-activating effect on the brain, which is based on the change of activity of the mesolimbic and nigrostriatal dopaminergic systems (activation of dopamine exchange in the nucleus accumbens and slowdown in the striatum). In behavioral tests, the polyprenolic preparation* showed the highest activity in the small (2.15 mg/kg) and medium (4.3 mg/kg) doses, whereas hepatoprotective properties of polyprenols* were more pronounced in the dose of 11.6 mg/kg. The latter were comparable with the action of heptral (300 mg/kg). Heptral based on lyophilisate had virtually no central effects. Heptral based on tablets aggravated the toxic effect of CC14, killing all the animals on the 7-th day of the experiment.

Conclusion

Polyprenols* have a pronounced hepatoprotective and neuroprotective effect in a wide range of doses. (Published in the proceedings of the 2nd Conference of the Russian Association of Psychoneuroendocrinology (RAPNE), Moscow, 2010)


* A polyprenolic preparation of Ropren was used the study, which is a pure concentrate of polyprenols (the total fraction is 95%). The text of the study is provided by courtesy of Solagran.